Molecular & Cellular Biologist

Company: Joul--
Location: Cambridge
Posted on: March 7, 2025

Job Description:

Job Title: Molecular & Cellular Biologist

Ready to make your application Please do read through the description at least once before clicking on Apply.
Location: Cambridge, MA
Type: Contract, Potential for extension or conversion to permanent

Responsibilities Follow existing lab standard operating procedures to:

Participate in sample collection of murine and human tissues, including the preparation of tubes, interfacing with in vivo scientists to retrieve samples, isolation of primary immune cells, and standard mammalian cell culture
Participate in sample preparation of murine and human tissues, including tissue fixation, dissociation, sectioning, and antibody staining
Generate spatial and single-cell data via a number of commercial workflows, including 10x Genomics and Parse Technologies
Interpret quality control metrics for spatial, single-cell and cytometry experiments


Requirements Masters + 3 years of experience in Biology, Immunology, or a related field Or Ph'D +0 yrs experience in Biology, Immunology, or a related field

3+ years of hands-on experience with standard immunoprofiling technologies, including

Flow Cytometry
Single-cell library preparation (ideally 10x Genomics or Parse Technology)
Preparation and loading of Illumina sequencing libraries
Bonus: generation of spatial transcriptomics data (any technology)

3+ years of experience with primary immune cells, including
Primary immune cell isolation
Maintenance of immune cell lines
Cell culture and in vitro stimulation of primary immune cells
Bonus: experience with CRISPR mediated functional genomics, including electroporation of primary immune cells

Benefits
System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.

Keywords: Joul--, Cambridge , Molecular & Cellular Biologist, Other , Cambridge, Massachusetts