Senior Research Associate I, Protein Purification and Characterization

Company: Takeda
Location: Cambridge
Posted on: November 20, 2023

Job Description:

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Job Description

About the role:

At Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on three therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.

Join Takeda as a Senior Research Associate I, Protein Purification and Characterization where you will produce therapeutics in the areas of oncology, neuroscience and gastrointestinal diseases, especially bispecifics/multispecifics and alternate/non antibody formats using multi column chromatography. You will also characterize purified proteins using suitable analytical techniques to enable isolation and identification of the required format. As part of the, Therapeutic Generation and Screening (TGS) team you will report to the Principal Scientist and support AI/ML approaches to develop the next generation of biotherapeutics, by working to innovate, enhance, and drive automated purification platforms and an IT infrastructure necessary to advance the biotherapeutic pipeline.

How you will contribute:

• To support Antibody Discovery, designs, purifies, and characterizes complex biotherapeutic molecules using optimal selection of chromatographic resins and binding assays to advance novel therapeutics.
• Conduct complex protein purification and characterization experiments from small scale to large-scale and initiates trouble shooting steps and automation to increase throughput when appropriate.
• Perform routine biosensor (SPR/BLI) based biomolecular interactions and data analysis.
• Exercise technical judgement in the interpretation of results and follow appropriate procedures to record data in internal databases and electronic lab notebooks.
• Communicate research and presents findings to internal stakeholders at team meetings.
• Maintain responsibility for laboratory instrumentation and supplies.
  
  Minimum Requirements/Qualifications:
  
  
  • MS in Biochemistry, Chemistry or Molecular Biology (or equivalent) with 1+ year experience.
  • B.S. in Biochemistry, Chemistry or Molecular Biology (or equivalent) with 3+ years of experience.
  • Prior experience developing methods for analytical SEC to enable identification of the test species and for BLI (Octet) or SPR (Biacore) to determine binding affinities and/or binding kinetics is a must.
  • Prior experience executing appropriate purification and characterization methods to purify bispecific/multispecific antibody like molecules, with a deep understanding of how different formats and mutations affect the purification process.
  • Prior experience purifying different formats using automated platforms to support AI/ML approaches to protein design is a plus.
  • Prior experience purifying and refolding proteins from bacteria is a plus.
  • Drive purification strategies including use of appropriate hosts to get the best protein and can guide CRO's using internally developed protocols. protocols.
  • Demonstrate an in-depth knowledge of protein characterization techniques, including CE-SDS, analytical SEC for purity and Octet/Biacore for determination of protein-protein interactions, binding kinetics, affinities, and epitope binning.
  • Recognize problems and consider the significance of irregularities within protein purification and characterization experiments.
  • Demonstrate a basic knowledge of workflows upstream (e.g., protein expression) and downstream (e.g., mass spec, cell based functional assays) of protein purification and how those workflows can affect the purification workflow.
    
    What Takeda can offer you:
    
    
    • Comprehensive Healthcare: Medical, Dental, and Vision
    • Financial Planning & Stability: 401(k) with company match and Annual Retirement Contribution Plan
    • Health & Wellness programs including onsite flu shots and health screenings
    • Generous time off for vacation and the option to purchase additional vacation days
    • Community Outreach Programs and company match of charitable contributions
    • Family Planning Support
    • Flexible Ways of Working
    • Tuition reimbursement
      
      More about us:
      
      At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs. Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work.
      
      Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.
      
      Base Salary Range: $89,000.00 to $117,000.00, based on candidate professional experience level. Employees may also be eligible for Short-term and Long-Term Incentive benefits as well. Employees are eligible to participate in Medical, Dental, Vision, Life Insurance, 401(k), Charitable Contribution Match, Holidays, Personal Days & Vacation, Tuition Reimbursement Program and Paid Volunteer Time Off. The final salary offered for this position may take into account a number of factors including, but not limited to, location, skills, education, and experience.
      
      In accordance with the CO Equal Pay Act, Colorado Applicants Are Not Permitted to Apply.
      
      #LI-SGM
      
      #LI-Onsite
      
      EEO Statement
      
      Takeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law.
      
      Locations
      
      USA - MA - Cambridge - Binney St
      
      Worker Type
      
      Employee
      
      Worker Sub-Type
      
      Regular
      
      Time Type
      
      Full time

Keywords: Takeda, Cambridge , Senior Research Associate I, Protein Purification and Characterization, Other , Cambridge, Massachusetts