Senior Research Associate I, Protein Purification and Characterization
Posted on: November 20, 2023
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About the role:
At Takeda, we are a forward-looking, world-class R&D
organization that unlocks innovation and delivers transformative
therapies to patients. By focusing R&D efforts on three
therapeutic areas and other targeted investments, we push the
boundaries of what is possible in order to bring life-changing
therapies to patients worldwide.
Join Takeda as a Senior Research Associate I, Protein Purification
and Characterization where you will produce therapeutics in the
areas of oncology, neuroscience and gastrointestinal diseases,
especially bispecifics/multispecifics and alternate/non antibody
formats using multi column chromatography. You will also
characterize purified proteins using suitable analytical techniques
to enable isolation and identification of the required format. As
part of the, Therapeutic Generation and Screening (TGS) team you
will report to the Principal Scientist and support AI/ML approaches
to develop the next generation of biotherapeutics, by working to
innovate, enhance, and drive automated purification platforms and
an IT infrastructure necessary to advance the biotherapeutic
How you will contribute:
- To support Antibody Discovery, designs, purifies, and
characterizes complex biotherapeutic molecules using optimal
selection of chromatographic resins and binding assays to advance
- Conduct complex protein purification and characterization
experiments from small scale to large-scale and initiates trouble
shooting steps and automation to increase throughput when
- Perform routine biosensor (SPR/BLI) based biomolecular
interactions and data analysis.
- Exercise technical judgement in the interpretation of results
and follow appropriate procedures to record data in internal
databases and electronic lab notebooks.
- Communicate research and presents findings to internal
stakeholders at team meetings.
- Maintain responsibility for laboratory instrumentation and
- MS in Biochemistry, Chemistry or Molecular Biology (or
equivalent) with 1+ year experience.
- B.S. in Biochemistry, Chemistry or Molecular Biology (or
equivalent) with 3+ years of experience.
- Prior experience developing methods for analytical SEC to
enable identification of the test species and for BLI (Octet) or
SPR (Biacore) to determine binding affinities and/or binding
kinetics is a must.
- Prior experience executing appropriate purification and
characterization methods to purify bispecific/multispecific
antibody like molecules, with a deep understanding of how different
formats and mutations affect the purification process.
- Prior experience purifying different formats using automated
platforms to support AI/ML approaches to protein design is a
- Prior experience purifying and refolding proteins from bacteria
is a plus.
- Drive purification strategies including use of appropriate
hosts to get the best protein and can guide CRO's using internally
developed protocols. protocols.
- Demonstrate an in-depth knowledge of protein characterization
techniques, including CE-SDS, analytical SEC for purity and
Octet/Biacore for determination of protein-protein interactions,
binding kinetics, affinities, and epitope binning.
- Recognize problems and consider the significance of
irregularities within protein purification and characterization
- Demonstrate a basic knowledge of workflows upstream (e.g.,
protein expression) and downstream (e.g., mass spec, cell based
functional assays) of protein purification and how those workflows
can affect the purification workflow.
What Takeda can offer you:
- Comprehensive Healthcare: Medical, Dental, and Vision
- Financial Planning & Stability: 401(k) with company match and
Annual Retirement Contribution Plan
- Health & Wellness programs including onsite flu shots and
- Generous time off for vacation and the option to purchase
additional vacation days
- Community Outreach Programs and company match of charitable
- Family Planning Support
- Flexible Ways of Working
- Tuition reimbursement
More about us:
At Takeda, we are transforming patient care through the development
of novel specialty pharmaceuticals and best in class patient
support programs. Takeda is a patient-focused company that will
inspire and empower you to grow through life-changing work.
Certified as a Global Top Employer, Takeda offers stimulating
careers, encourages innovation, and strives for excellence in
everything we do. We foster an inclusive, collaborative workplace,
in which our teams are united by an unwavering commitment to
deliver Better Health and a Brighter Future to people around the
Base Salary Range: $89,000.00 to $117,000.00, based on candidate
professional experience level. Employees may also be eligible for
Short-term and Long-Term Incentive benefits as well. Employees are
eligible to participate in Medical, Dental, Vision, Life Insurance,
401(k), Charitable Contribution Match, Holidays, Personal Days &
Vacation, Tuition Reimbursement Program and Paid Volunteer Time
Off. The final salary offered for this position may take into
account a number of factors including, but not limited to,
location, skills, education, and experience.
In accordance with the CO Equal Pay Act, Colorado Applicants Are
Not Permitted to Apply.
Takeda is proud in its commitment to creating a diverse workforce
and providing equal employment opportunities to all employees and
applicants for employment without regard to race, color, religion,
sex, sexual orientation, gender identity, gender expression,
parental status, national origin, age, disability, citizenship
status, genetic information or characteristics, marital status,
status as a Vietnam era veteran, special disabled veteran, or other
protected veteran in accordance with applicable federal, state and
local laws, and any other characteristic protected by law.
USA - MA - Cambridge - Binney St
Keywords: Takeda, Cambridge , Senior Research Associate I, Protein Purification and Characterization, Other , Cambridge, Massachusetts
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