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Research Technologist

Company: Massachusetts General Hospital(MGH)
Location: Cambridge
Posted on: June 23, 2022

Job Description:

GENERAL SUMMARY/ OVERVIEW STATEMENT:The Yu Laboratory at the Ragon Institute of MGH, MIT and Harvard at the Massachusetts General Hospital in Boston has an opening for a highly motivated Research Technologist to study the immunopathogenesis of HIV infection and viral reservoirs in HIV elite controllers. The overall goal is to conduct human translational research to understand the immune control mechanisms of HIV. The person hired will work under the supervision of Dr. Xu Yu conducting patient-oriented translational research involving cutting-edge technologies. The position will involve handling and processing of blood samples, performance of molecular biological and immunologic assays, including nucleic acid extractions, PCR and next generation sequencing, and utilizing novel assays to study viral persistence. The position will also involve general lab organization and assistance with lab logistics.This is a great opportunity for someone interested in pursuing a career in scientific research or medicine while working in a supportive, highly collaborative, and energetic environment. The lab studies samples from ongoing clinical cohorts based in the US as well as multiple countries in Europe, Africa, and South America, examining risk of HIV, HIV pathogenesis, and other adverse reproductive outcomes. State-of-the-art facilities are available within an exceptionally energetic research setting. The Yu lab is committed to providing a diverse, inclusive, and supportive environment; appropriately qualified applicants from all backgrounds will be considered.PRINCIPAL DUTIES AND RESPONSIBILITIES:Perform experiments analyzing blood and tissue samples from human subjects, assessing the viral reservoir and immune responses to HIV infection Perform and assist with experiments involving immunologic assays, flow cytometry and molecular biology Perform assays utilizing standard molecular biology and immunology techniques (cloning, DNA and RNA extraction, quantitative PCR, droplet digital PCR, reverse transcription, gel electrophoresis, flow cytometry, library preparation for next generation sequencing)Assist in cell/tissue processing and in vitro cell culture maintenance in a BSL-2+ settingMaintain detailed documentation of experimental workWork closely with other lab members as well as with outside collaboratorsPerform data analysis and visualization in Microsoft Excel, GraphPad Prism, and Adobe IllustratorEnsure quality control of equipment, reagents, and cellular components and participate in ongoing quality control/assurance programsHelp support lab organization and logisticsMaintain close communications with PI regarding progressPresent findings in weekly one-on-one meetings with the PI and on a quarterly basis in lab meetingsQualifications SKILLS/ABILITIES/COMPETENCIES REQUIRED:Highly motivated and independent, with the ability to work in a dynamic team environmentExcellent organizational skills and attention to detailLaboratory experience requiredFlexibility to occasionally work evenings or weekendsExperience with flow cytometry, next generation sequencing, cell culture strongly preferredComputer experience including expertise with Microsoft Office programs is essentialExperience with R is preferred but not essentialEDUCATION:Bachelor's degree required. EXPERIENCE: Several years of directly related work experience in a scientific laboratory is strongly preferred.EEO Statement Massachusetts General Hospital is an Equal Opportunity Employer.By embracing diverse skills, perspectives and ideas, we choose to lead. Applications from protected veterans and individuals with disabilities are strongly encouraged.

Keywords: Massachusetts General Hospital(MGH), Cambridge , Research Technologist, Other , Cambridge, Massachusetts

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