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Genomics Scientist

Company: Pfizer
Location: Cambridge
Posted on: September 16, 2020

Job Description:

ROLE SUMMARY

We are seeking an individual to join the Pfizer NGS Technology Center. This individual will interface with a diverse set of colleagues to support the application of NGS technologies to the portfolio projects across all active therapeutic areas from preclinical to clinical samples. This individual will carry-out all laboratory in-house NGS assay experimentation and development including the design, execution of laboratory experiments, validation of test data, as well as preparation of associated documentation and reports. This person will also carry out upstream QC and initial processing of NGS data before handing off to computational biologist for downstream processing. The successful candidate must be technically skilled to perform sample collection/ handling, tissue dissociation, isolation of RNA or DNA from tissues and cells and be excited to inquire about and apply the latest and most cutting-edge molecular biology technologies. Meticulous attention to detail, proficient reasoning and problem-solving skills in experimental design are a must.

ROLE RESPONSIBILITIES


  • Perform and maintain competency in complex Next Generation Sequencing assays/tasks according to established operational procedures under supervision.
  • Familiarity with automation workflows on robotic NGS equipment.
  • Carries out all lab-based activities: sample preparation, library construction, data generation, upstream data processing to ensure high level of quality and reliability of generated results.
  • Effectively optimizing and troubleshooting NGS library preparation.
  • Application of Bioanalyzer and other nucleic acid analysis techniques to library construction quality control.
  • Serves as a subject matter expert for NGS technology, provides guidance on experimental design, method or platform selection, and data management to address a specific need.
  • Educates other scientists and provides hands-on training of tech center equipment and delivers internal presentations when applicable.
  • Keeps current and stays at the cutting edge of various NGS platforms and technical innovations.
  • Drafts experimental plans, validation reports, work instructions and SOPs.

    QUALIFICATIONS

    Education and Experience

    • Bachelor's degree and previous experience that provides the knowledge, skills, and abilities to perform the job (comparable 6+ year)
    • Master's degree and previous experience that provides the knowledge, skills, and abilities to perform the job (comparable 6+ year)

      Knowledge, Skills and Abilities

      • Must have at least 1-2 years of wet lab experience with NGS assay development, validation, troubleshooting, and reviewing of run QC data.
      • Knowledge of different NGS platforms (RNA Sequencing, targeted RNASeq, amplicon-based sequencing, Chipseq and or other NGS assays).
      • Experience with plate-based robotic automation platforms for nucleic acid isolation, QC, library preparation is highly desired but not required.
      • Experience in DNA/RNA extraction, nucleic acid quantitation and quality control analysis.
      • Perform genetic and molecular biology laboratory procedures involving traditional techniques such as PCR, isolation of DNA and RNA, DNA Fragment Analysis, DNA and RNA sequencing, RT-PCR, Real-Time PCR, and other molecular techniques
      • Must be self-motivated and able to learn quickly.
      • Must be goal oriented and focused on completing work on time.
      • Must be fluent in English both oral and written and possess good communication skills.
      • Full understanding of laboratory requirements and SOPs.

        #LI-PFE

        Other Job Details:

        • Eligible for Employee Referral Bonus


          Sunshine Act

          Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.EEO & Employment Eligibility

          Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.Research and Development

Keywords: Pfizer, Cambridge , Genomics Scientist, Other , Cambridge, Massachusetts

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