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Associate Scientist

Company: Intellia Therapeutics
Location: Cambridge
Posted on: March 28, 2020

Job Description:

Associate Scientist Duties: Purify Cas9, Cas9 variants, Cas9-fusions, and Cas family proteins. Perform biochemical analysis to test for activity of Cas9. Purify various other protein reagents. Analyze proteins by gel, SEC-MALS, LAL, and A280. Perform cell based assay. Participate in research collaboration with other groups within Intellia. Form RNP for preclinical studies. Perform early stage development experiments for Cas9 production and purification. Evaluate new technologies and approaches to support Intellia's genome editing platform. E. coli expression of various proteins. Requirements : Requires a Bachelor's degree in Chemistry, Biochemistry, Biology, Biotechnology and Chemical Science or a directly related field plus four (4) years of industry experience.

  • Two (2) years of experience in the following:
    • Experience with protein purification, characterization, or HPLC analysis
    • Knowledge of affinity purification techniques, including immobilized metal chelation affinity chromatography (IMAC) for Hisx6 tagged proteins or protein affinity tags such as streptavidin tags (use of StrepTactin resin)
    • Knowledge and application of the primary protein structure, such as protein isoelectric point, and developing protein purification methods, such as cation or anion exchange
    • Expressing protein in E. coli cells
    • Running electrophoresis methods such as SDS-PAGE or western blots One (1) year of experience in the following:
      • Performing analytical size exclusion chromatography (SEC-HPLC) with multi-angle light scattering (MALS) to measure molecular weight Will accept a Master's degree plus two (2) years of industry experience in lieu of Bachelor's degree plus four (4) years of industry experience. 40 hours per week. Day shift. What Intellia Stands For: At Intellia, our mission is to develop potentially curative genome editing treatments that can positively transform the lives of people living with severe and life-threating diseases. We push boundaries. We are focused on positively disrupting health care treatment of patients with genomic diseases. The values we live by and drivers we look for are:
        • One is respect for individuals; our unique qualities and strengths; our own ways to understand, learn and improve. One is our single-minded determination to excel; to succeed together. One is you and us - trusting and counting on every single one of us.
        • Explore because we seek new ways to tackle disease. Explore with unbounded minds...free from assumptions, open to ideas. Explore by staying curious, by pushing boundaries, overcoming obstacles. Explore means thinking of new ways.
        • Disrupt by thinking courageously...and creating a better future. Disrupt by defying conformity; interrogating the status quo; questioning our momentum. Disrupt with challenging thoughts...with that breed of skepticism that shows you have better solutions.
        • Deliver with your focus on the objective and your determination to complete the task. Deliver by advancing relentlessly and by maintaining high standards...even when nobody is watching. Deliver by staying accountable and pulling your weight. Deliver because patients are counting on us to make the promise reality. Get it done. POSITION: Full-Time, Exempt EEOC Statement: Intellia believes in a diverse environment, and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race, color, religion, gender, national origin, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Intellia will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.

Keywords: Intellia Therapeutics, Cambridge , Associate Scientist, Other , Cambridge, Massachusetts

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